CD8A and neoplasm: At this time point, there was no significant difference in TIM-3 expression between treated and untreated tumours in both EGFR and Kras mice; however, a significant increase in interferon-gamma-positive CD8 T cells was observed (Fig. 2b, Supplementary Fig. 1a,f) suggesting that TIM-3 elevation was not simply correlated with T-cell activation.