MLH1 and colorectal carcinoma: By contrast, CIMP-H CRC without MLH1 methylation (n = 32) demonstrated significantly higher frequencies of distant metastasis (34%; P = 0.033), vascular invasion (28%; P = 0.006), perineural invasion (47%; P = 0.006), and tumor budding (81%; P = 0.011) (Table 1).