Next, to evaluate whether TRAF3–TAK1 interaction is required for the regulatory function of TRAF3 on hepatic steatosis, an adenovirus harbouring TRAF3 with a mutant 267–376 aa domain (AdTRAF3-M) was constructed and injected into WT mice in parallel with AdTRAF3 at 20 weeks of HFD administration. The gene discussed is TRAF3; the disease is Hepatic steatosis.