Given that hepatocytes are the primary cell type that is damaged during hepatic steatosis, in our present study, hepatocyte-specific TRAF3-KO and TRAF3-transgenic mice are generated and subjected to an high-fat diet (HFD) to evaluate the effects of TRAF3 on hepatic steatosis and related metabolic disorder. The gene discussed is TRAF3; the disease is Hepatic steatosis.