HIF1A and melanoma: Consistent with the findings from an independent study that reported an siRNA screen in melanoma cells [9], our results revealed that silencing of the three DNA damage response (DDR) factors—ATM, checkpoint kinase 1 (CHEK1) and checkpoint kinase 2 (CHEK2)— led to altered HIF-1α-NanoLuc expression levels under hypoxic conditions and the three genes are involved in several signaling pathways and networks identified by the siRNA screen (Table S3 and S4).