Consistent with the findings from an independent study that reported an siRNA screen in melanoma cells [9], our results revealed that silencing of the three DNA damage response (DDR) factors—ATM, checkpoint kinase 1 (CHEK1) and checkpoint kinase 2 (CHEK2)— led to altered HIF-1α-NanoLuc expression levels under hypoxic conditions and the three genes are involved in several signaling pathways and networks identified by the siRNA screen (Table S3 and S4). The gene discussed is CHEK1; the disease is melanoma.