Next, we rationally narrowed the list down using the following criteria: a) miRNAs under-expressed in TAL or LMO overexpressing cases (TAL/LMO cytogenetic subgroup, based on primary T-ALL gene expression profiling [23]); b) concomitant identification of LMO2 as putative target, given the frequent aberrant co-expression of both TAL1 and LMO2; c) more than one predicted target site in the 3′UTR of the TAL1 mRNA and/or 8mer (or 9mer) type of seed paring; d) identification of the miRNA as regulator of TAL1 expression by at least two different algorithms. This evidence concerns the gene LMO2 and acute lymphoblastic leukemia.