As the strongest down‐regulation of expression by miR‐515‐5p was seen with MARK4 in both MCF7 and MDA‐MB‐231 and that this gene had previously been reported to modulate NSCLC cell motility 16, we decided to focus our study on investigating the direct regulation of MARK4 expression by miR‐515‐5p. The gene discussed is MARK4; the disease is non-small cell lung carcinoma.