These four transcripts were also down‐regulated in A549 and H1299 NSCLC cells in response to miR‐515‐5p overexpression and luciferase reporter constructs, indicating that miR‐515‐5p directly regulated NRAS, PIK3C2B and MARK4 expression in multiple cancer cells. This evidence concerns the gene PIK3C2B and non-small cell lung carcinoma.