We also found that EpCAM+ HCC cell organoids rely on the presence of EpCAM and β-catenin signaling since RNAi-mediated silencing of EpCAM or small molecular inhibitors of wnt-β-catenin signaling, identified from a high-throughput screen of small molecule libraries from the Developmental Therapeutics Program of NCI25, can effectively inhibit organoid formation. Here, EPCAM is linked to hepatocellular carcinoma.