TGFB1 and hepatocellular carcinoma: Histological analysis revealed that while the frequency of HCC occurrence and the formation of visible tumors in the liver was similar in each treatment group, (Fig. 4C, panels i and ii; Suppl Table 2), the numbers of macroscopic nodules detected in the liver was significantly higher in mice from 3D cells treated with TGF-β, whereas TGF-β had a less significant effect on the number of nodules detected in tumors from 2D cells (Fig. 4C, panel i vs. panel ii, Fig. 4D).