Although this study provided no direct evidence to show that FASN activation is indispensable for HFD-induced enhancement of the LNCaP xenograft tumour growth, the higher level of P-AKT, P-ERK, Ki67 positivity and FASN in the HFD xenograft may support the results of in vitro experiments and consequently the significance of FASN. The gene discussed is AKT1; the disease is neoplasm.