To assess the expression levels of TFE3, HIF-1α, PAI-1, and EGFR, tumor sections from human tissue arrays for HNSCC (n = 59) were stained with antibodies against TFE3, HIF-1α, PAI-1, and EGFR compared with oral mucosa (n = 39) and lymph node metastasis (n=5). This evidence concerns the gene SERPINE1 and head and neck squamous cell carcinoma.