Considering the observed increased production of APC-derived pro-inflammatory cytokines in plasma of pemphigus patients, we next sought to investigate whether the polarization of certain CD4+ T cells is affected by analyzing the production of T cell signature cytokines (IFN-γ, IL-4 and IL-10) with flow cytometry (Gating: S3 Fig). The gene discussed is IFNG; the disease is pemphigus.