The specificity for item 1 was slightly lower (81.2%) than that for the other items because all types of CRC, including the sporadic type, occurred at a younger age recently.23 In the present study, 7.1% of patients did not check for IHC staining for hMLH1 and hMSH2, 21.8% of patients did not check for MSI. This evidence concerns the gene MSH2 and colorectal carcinoma.