Prominent genes in these pathways were granzyme A (GZMA), granzyme B (GZMB), perforin 1 (PRF1), killer cell lectin-like receptor subfamily D, member 1 (KLRD1), CD8A and CD8B. Under-expressed pathways included primary immunodeficiency signaling, hematopoiesis from pluripotent stem cells, B cell development and androgen signaling (Fig 2c). Here, PRF1 is linked to inborn error of immunity.