As shown in Figure 2F–2G, PC14 cells co-cultured with RNase A/T-treated astrocytes-22bpCy3 acquired a large fraction of the synthetic sRNA, which did not differ significantly from the MFI values of Cy3 fluorophore in the PC14 cells / astrocyte-22bpCy3 co-cultures that were not treated with RNase A/T (Figure 2H; mean ± SEM, 9.3 ± 0.9 and 7.8 ± 2.3, respectively To further assess the generality of sRNA transfer from astrocytes to cancer cells, we used the same procedure to examine the transfer of 22bpCy3 from astrocytes to MDA-MB-231 cancer cells. This evidence concerns the gene RNASE1 and cancer.