EZH2 and malignant colon neoplasm: Therefore, to determine if EZH2 is involved in the regulation of miR-31 expression through histone H3K27me3, we examined H3K27me3 levels around the transcription start site on the miR-31 promoter by performing ChIP assay and found that EZH2-mediated histone methylation downregulates miR-31 expression in colon cancer.