To further explore the association between β-catenin activity and the area of KCNQ1OT1 lncRNA-coated territory, we performed knockdown of β-catenin transcription using short interfering RNA (siRNA) in the HCT15 cells that showed the highest amount of nuclear β-catenin protein of the colorectal cancer cell lines tested (Fig. 1C). Here, KCNQ1OT1 is linked to colorectal cancer.