To address the role of HDAC9 in ischaemic stroke in vivo, we infused pGLV3‐shRNA‐HDAC9 into the left cerebral cortex of rats by stereotaxic injection as shown in Figure 2A. After 1 week injection, intense green fluorescence was seen in the cerebral cortex after delivery of the lentivirus pGLV3‐shRNA‐HDAC9 encoding GFP, but not in control brains in rats injected with PBS. This evidence concerns the gene HDAC9 and ischemic stroke.