Further investigations providing one explanation for the inhibitory effect of 5-Fu/P85 copolymer micelles on colon cancer, showed that 5-Fu/P85 copolymer micelles could decrease the content of CD133 + CXCR4+ cancer cells in HCT116 and RKO cell lines and suppress EMT of CD133 + CXCR4+ cancer cells. This evidence concerns the gene PROM1 and colonic neoplasm.