As shown in Fig. 6B, we also detected the mRNA expression of the above EMT-related genes in the mice colon cancer metastatic liver tissues, and the results showed that compared with mice tissues from control groups, the tissue from the group administered with 5-Fu/P85 copolymer micelles had higher E-cadherin expression and lower vimentin, snail and twist expression, which was consistent with the result in Fig. 6A. This evidence concerns the gene TWIST1 and malignant colon neoplasm.