The absence of the variant allele in tumor cell recoveries indeed is coherent with the observation of the LOH event in the same KDR gene, revealed by two additional germline heterozygous SNPs in the stromal cells, flipping in tumor cells to either 0% or 100% variant in tumor cells (Fig. 3a row 14 and row 6, respectively). The gene discussed is KDR; the disease is neoplasm.