To evaluate the effects of OR3A4 on the biological behavior of gastric cancer cell lines, we constructed a stable pcDNA-OR3A4 expression vector and a stable pSilencer-OR3A4 small-interfering RNA (siRNA) vector and transfected gastric cancer cell lines SGC-7901 and NCI-N87 with these constructs. This evidence concerns the gene ENSG00000290820 and gastric cancer.