GAS5 and breast carcinoma: In initial experiments, we therefore examined if DNA oligonucleotides based on the wild-type GAS5 HREM sequence alone also induced apoptosis in a range of breast cancer cell lines; negative controls were nucleofected with DNA oligonucleotides either with stem complementarity but lacking the GAS5 HRE consensus (stem loop or SL control) or with scrambled GAS5 sequence (Scram control).