Due to the very low baseline expression of Auxilin-1 mRNA levels, we focused our studies on Auxilin-2 and verified the upregulation of Auxilin-2 mRNA levels in Ph+ ALL and CML cell lines treated with either Imatinib, or the 2nd generation TKI, Nilotinib (Fig. 2f, Additional file 2: Figure 2b). This evidence concerns the gene GAK and chronic myelogenous leukemia, BCR-ABL1 positive.