For example, following therapeutic vaccination with a replication-incompetent recombinant human adenovirus serotype 5 (rHuAd5) vector expressing a relevant tumour-associated antigen (TAA) in tumour-bearing animals, microarray analysis uncovered the progressive appearance of immunosuppressive genes in the tumour microenvironment, including Pdcd1 (PD-1), HAVCR2 (TIM-3) and LAG3 (CD223). Here, LAG3 is linked to neoplasm.