ANKRD1 and infection: Figure 6D showed that knockdown of ANKRD1 displayed no effect on HCV protein levels in Jc1 infected cells (upper panel). To further analyze the possible involvement of ANKRD1 in virion production, naïve Huh7.5 cells were infected with Jc1 harvested from the supernatant of the 1st infection. Figure 6D showed that HCV protein levels were not altered in cells re-infected with Jc1 from ANKRD1 knockdown cells (lower panel).