We used lean WT (C57BL/6J) male mice as a control group, C57BL/6J male mice with diet induced obesity (WT-Obese) as a non-transgenic model of cardiac deficiency [18–20, 78], and calsequestrin 2 (cardiac isoform) deficient (Casq2-/-) male mice as a genetic model with reported cardiac deficiency [23, 75] (S1 Table). The gene discussed is CASQ2; the disease is obesity due to melanocortin 4 receptor deficiency.