KRAS and infection: Under the assay conditions described herein, IFA revealed that the maximum level of LGTV infection of the ISE6 cell population (>96%) corresponded to an MOI of 10 as determined by percentage of cells labeled with the LGTV NS3 protein (Fig 1A and 1B), and plaque assays revealed that the peak of LGTV release from ISE6 cells occurred at 36 hpi (Fig 1C).