Real-time PCR on samples from iLgr5;GLI2A gastric tumors revealed upregulation of mRNA encoding multiple EMT markers, including Snail1 (8.1-fold), Snail2/Slug (2.5-fold), FSP1 (3.6-fold), and Twist1 (3.9-fold) (Figure 3E). This evidence concerns the gene SNAI2 and gastric neoplasm.