As the downregulation of Atg9 and Lamp1 in Bif-1 KO adipose tissue was only observed in 30-week-old but not 6-week- or 10-week-old animals (Fig. 6d, Supplementary Fig. S5), this effect appears to be a consequence of the development of obesity in Bif-1 KO mice. The gene discussed is LAMP1; the disease is obesity due to melanocortin 4 receptor deficiency.