Furthermore, to measure the expression levels of RNF135 in different pathological grade gliomas and normal brain tissues, we examined the RNF135 expression levels in 154 archived paraffin-embedded samples, including 14 astrocytic tumours, 17 oligodendroglioma, 66 anaplastic astrocytoma and 45 glioblastoma and 12 NB tissues, using the immunohistochemical (IHC) staining assay (Fig. 1C). This evidence concerns the gene RNF135 and central nervous system cancer.