In this exploratory set, no difference in the methylation patterns between PCa and BPH 39 PCa-associated changes when compared to healthy controls; 7 out of them were confirmed by sequencing, including RNF219 Diagnostic performances of RNF219: Se = 89%, Spe = 71%, AUC = 0.79 The gene discussed is OBI1; the disease is benign prostatic hyperplasia.