Experimental studies on time-dependence of HMGB1 level elevation showed increased levels in a rat model with glioblastoma multiforme five days after treatment with adenoviral vectors [203], and elevated extracellular HMGB1 levels 48 h after in vitro treatment of glioma, melanoma and lung carcinoma cells with temozolomide [146]. This evidence concerns the gene HMGB1 and glioma.