RNASE3 and Increased total eosinophil count: Subjects in cluster F (mixed granulocytic inflammation with severe obstruction) have both sputum eosinophilia and neutrophilia with lower prebronchodilator FEV1 values and FEV1/forced vital capacity ratios associated with higher sputum periostin and eosinophil cationic protein (ECP) levels and high HAD scores.