To assess the impact of HPSE on the expression of epithelial marker E-cadherin and mesenchymal markers vimentin and fibronectin in MM cells, cellular protein was isolated from HPSE-low (human MM CAG cells transfected with empty vector) and HPSE-high (CAG cells transfected with human HPSE cDNA) MM cells [9, 14], and E-cadherin, Vimentin and fibronectin expression was examined by Western blotting. Here, FN1 is linked to Miyoshi myopathy.