To further determine the relationship between HPSE expression and the expression of epithelial marker and mesenchymal marker in different MM cell lines, wild-type CAG and RPMI 8226 human MM cell lines were cultured in the absence or presence of recombinant human HPSE (rhHPSE) for 48 hrs, E-cadherin and vimentin expression were assessed by Western blot. This evidence concerns the gene VIM and Miyoshi myopathy.