Endothelial-specific Chd1 mutants, while able to produce intact E10.5 IAHC, that show intermediate markers of differentiation such as RUNX1 and c-Kit, do not successfully initiate definitive hematopoiesis and die by E15.5 due to severe anemia and absolute failure of erythropoiesis [207]. Here, KIT is linked to anemia (phenotype).