To measure cf-DNA, a major constituent of NETs and cf-DNA associated with MPO (cf-DNA/MPO), another constituent of NETs, we used two methodologies and demonstrated that, in addition to the levels of free DNA (cf-DNA) being higher in relation to the levels of cf-DNA/MPO in the serum, both methods show similar results for the release after sepsis induction. This evidence concerns the gene MPO and Sepsis.