Zoledronate inhibited cell-matrix and cell-cell interactions, migration potential, and the invasive activity by induction of E-cadherin and reduction of N-cadherin with activation of transcription factors Slug and Twist in 4 osteosarcoma cells, especially in U2OS cells and seemed to possess the most potency in U2OS cells. The gene discussed is CDH2; the disease is osteosarcoma.