To determine whether pH1N1 can promote death through Fas-mediated apoptotic pathway in Jurkat cells, total cell lysates from cells infected with HIV-1 followed by infection with pH1N1 for an additional three days were used for IP with anti-Fas antibody and Western blotting with anti-caspase-8 antibody to evaluate death-inducing signaling complex (DISC) formation. This evidence concerns the gene CASP8 and infection.