In contrast, no Pitx2 nuclear staining was observed in 59 of the 92 well-differentiated adenocarcinoma (WD-PDAC) tissues and in 131 of the 164 moderately- or poorly-differentiated adenocarcinoma (MD-PDAC or PD-PDAC) tissue samples (Figure 1D and 1E). This evidence concerns the gene PITX2 and adenocarcinoma.