Hypermethylation at KLF4 gene promoter has been observed in gastric carcinoma cells (−130 ~ −13 region; ref. 14) and in colorectal carcinoma cells (+79 ~ +355 region; ref. 13), but the − 481 ~ +192 region was not methylated in this study. This evidence concerns the gene KLF4 and colorectal carcinoma.