To further evaluate the concept of a TGF-β1 dependent myofibrosis in PAD, we selected three adjacent gastrocnemius sections and stained the first section for collagen with Masson Trichrome, the second for TE-7 positive fibroblasts by immunohistochemistry, and the third for TGF-β1 by immunofluorescence. This evidence concerns the gene TGFB1 and peripheral arterial disease.