Focusing on genes previously shown to be recurrently mutated in CLL; at TP2, clonal mutations were detected in MYD88 (p.L265P; pt-2), and CHD2 (pt-1) among the three untreated patients and in ATM (pt-4), DDX3X (pt-13), NOTCH1 (pts-6,13), SF3B1 (pts-6, 8), TP53 (pts-8, 9), NFKBIE (pt-5), SPEN (pt-9), ZMYM3 (pt-6), KLHL6 (pt-10), BIRC3 (pt-13) and IRF4 (pt-13) among the 10 patients who received treatment. This evidence concerns the gene SPEN and B-cell chronic lymphocytic leukemia.