As shown in Figs. 1a, b; 2a, b; 3a, b and 4a, b, positive staining rates of DLC-1, Rho A, ROCK2 and moesin in liver cancer tissue samples were 38.75, 78.75, 75.00 and 86.25 % respectively, which was significantly different from those in normal mucosa (83.75, 43.75, 40.00 and 66.25 %) (P < 0.001, Table 1). The gene discussed is DLC1; the disease is liver cancer.