The results were further biologically validated using multiple lung SCC cell lines, suggesting that our approach using gene expression profiles of the 105 NSCLC cell lines provide statistical power to identify potential genes correlated with SOX2. In addition to determining SOX2 downstream genes in lung SCC cell, our approach will be useful for identifying genes regulated by SOX2 in other cancer cells or genes regulated by any other transcription factors in multiple cancer cells. The gene discussed is SOX2; the disease is cancer.