Hence, shNC, sh32b#2 and sh32b#2/GFP-ANP32B-infected MDA-231-D3H2LN cell line (Figure 2e), which was derived from breast cancer cell line MDA-MB-231 with stable luciferase expression, were injected into the mammary fat pad of nude mice. The gene discussed is ANP32B; the disease is breast carcinoma.