On the other hand, the “unbiased” approach involves the use of modern techniques including proteomics, metabolomics, and bioinformatics that have allowed unbiased investigations of numerous putative markers that may be informative with regard to the various stages of NAFLD, including overt NASH and its sequelae.44 In order to construct our panel, we selected the biomarkers with the high evidence for their role in NAFLD (ie, adiponectin, visfatin, resistin, hepcidin, IL-6, IL-8, and TNF-alpha) using a “knowledge-based” approach. Here, RETN is linked to metabolic dysfunction-associated steatohepatitis.