Our bioinformatics software prediction showed that miR-490-3p has a direct target in the 3′ UTR of TGFα, our dual luciferase reporter assays, the mRNA and protein expression levels of TGFα in miR-490-3p-transfected cells and in nude mice the tumor tissues of Hsa-490-3p group were decreased all verified this prediction. This evidence concerns the gene TGFA and neoplasm.