Here, we showed that physalin A inhibited the phosphorylation of JAK2 and JAK3 in human NSCLC cells with minimal impact on that of JAK1, TYK2 and Src, suggesting that the inhibition of Tyr705-p-STAT3 was mediated by JAK2 and JAK3 inhibition. Here, JAK3 is linked to non-small cell lung carcinoma.