During chronic continuous dosing with DSS, Tff2-null mice died earlier than both wild-type and CD2–TFF2 animals, but there were no differences in body weight loss during acute colitis, and little difference in cytokine levels, except for a higher colonic interleukin-1β (IL-1β) and IL-6 level in Tff2-null mice, with lower levels in CD2–Tff2 mice (Supplementary Fig. 3a–d). This evidence concerns the gene TFF2 and colitis.