CNR1 and neoplasm: On the other hand, an in vitro study with Caco-2 cells has also shown possible anticancer effects for EVOO by means of this mechanism, since it influenced DNA methylation state at CNR1 promoter which was inversely correlated to selective and transient up-regulation of CNR1 gene, a tumor suppressor that encodes for type 1 cannabinoid receptor (CB1).