According to our results and expectations, HMGB1 was over-expressed in NSCLC tissues, and some of the reasons that may lead to elevation of HMGB1 in serum and tissues of NSCLC patients might include the secretion of HMGB1 by lung cancer cells and that the expression of HMGB1 increased in primary ADC tissues compared to non-cancerous tissues [43]. This evidence concerns the gene HMGB1 and lung cancer.