Two hour pulse treatment of SKOV3 ovarian cancer cells with Trast-SA targeted nanoparticles containing GAPD siRNA resulted in greater reduction of GAPD expression at 48 hours compared to BHV1-SA as assessed by quantitative RT-PCR (Figure 3a) and GAPD enzyme activity (Supplementary Figure S1b). Here, GAPDH is linked to ovarian carcinoma.